Abrogation of hepatitis C virus NS3 helicase enzymatic activity by recombinant human antibodies
نویسندگان
چکیده
منابع مشابه
Fuel specificity of the hepatitis C virus NS3 helicase.
The hepatitis C virus (HCV) NS3 protein is a helicase capable of unwinding duplex RNA or DNA. This study uses a newly developed molecular-beacon-based helicase assay (MBHA) to investigate how nucleoside triphosphates (NTPs) fuel HCV helicase-catalyzed DNA unwinding. The MBHA monitors the irreversible helicase-catalyzed displacement of an oligonucleotide-bound molecular beacon so that rates of h...
متن کاملStructure-based mutagenesis study of hepatitis C virus NS3 helicase.
The NS3 protein of hepatitis C virus (HCV) is a bifunctional protein containing a serine protease in the N-terminal one-third, which is stimulated upon binding of the NS4A cofactor, and an RNA helicase in the C-terminal two-thirds. In this study, a C-terminal hexahistidine-tagged helicase domain of the HCV NS3 protein was expressed in Escherichia coli and purified to homogeneity by conventional...
متن کاملHepatitis C Viral NS3-4A Protease Activity Is Enhanced by the NS3 Helicase*S⃞
Non-structural protein 3 (NS3) is a multifunctional enzyme possessing serine protease, NTPase, and RNA unwinding activities that are required for hepatitis C viral (HCV) replication. HCV non-structural protein 4A (NS4A) binds to the N-terminal NS3 protease domain to stimulate NS3 serine protease activity. In addition, the NS3 protease domain enhances the RNA binding, ATPase, and RNA unwinding a...
متن کاملMultiple enzymatic activities associated with recombinant NS3 protein of hepatitis C virus.
The hepatitis C virus (HCV) nonstructural 3 protein (NS3) contains at least two domains associated with multiple enzymatic activities; a serine protease activity resides in the N-terminal one-third of the protein, whereas RNA helicase activity and RNA-stimulated nucleoside triphosphatase activity are associated with the C-terminal portion. To study the possible mutual influence of these enzymat...
متن کاملDiscovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide.
Worldwide, over 170 million people have hepatitis C virus (HCV) infections. Chronic infection with HCV leads to liver diseases such as cirrhosis and hepatocarcinoma and is the major reason of liver transplantation. Currently, the standard treatment for hepatitis C relies on a combination of interferon-a and ribavirin (an immune booster and a general inhibitor of virus replication, respectively)...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2003
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.19299-0